Shanghai Kekai Biotechnology Co., Ltd.

Product name: Protein A, Recombinant Protein A, Protein A

CAS number: 91932-65-9

Product keywords: Protein A Protein A Recombinant Protein A Staphylococcus aureus protein A

Product category:Chemical chemical reagents Biochemical reagents

Grade: Chromatographically pure

Usage: Other

Content: Standard product

Application: Medicine

Model: KK-T005

Specifications: mg, g

Trademark: Kekai-Bio

Packaging: mg, g

Product Description:

Source: Tritirachium album limber

Molecular weight: Theoretical MW: 28.9 kDA

Active unit definition: The amount of proteinase K per minute that hydrolyzes the substrate casein to produce 1 μmol of tyrosine at 37 °C and pH 7.5, defined as one unit (U).

Storage: The dry powder state can be stored at low temperature under the condition of 2~8 °C. After dissolution, it is divided into an appropriate volume and stored at -20 °C.

Stability: Transportation can be carried out at room temperature.

Product Introduction

Form: lyophilized powder

Ingredients: Recombinant protein A aqueous solution is directly lyophilized without any other additives.

Purity (SDS-PAGE detection): >95%

Nucleic acid (UV detection): no detection of DNA, RNA

Triton content: No Triton pollution

Storage: 2°C~8°C

pH stability range: 1-13

source

Recombinant protein A is expressed by recombinant E. coli and purified by HPLC.

Product advantages

1. Non-antibody column purification, no contamination of any other antibodies such as IgG in the product.

2. SPA-25 is a product independently developed by the company, with a wide pH tolerance range, which can withstand 0.5 M NaOH and 0.5M HCl treatment, non-degradation, and the antibody binding ability remains unchanged.

3. The product has a linker specially designed for antibody purification column, which can be directly used in the preparation of protein A column.

4. The company's recombinant protein A products have the advantages of large production scale, stable quality and high purity.

Product use

Recombinant protein A can be coupled to a solid separation medium such as agarose for purification of monoclonal or polyclonal antibodies. Recombinant protein A can be coupled to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radiolabeling, etc.) without affecting its activity to bind to antibodies. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry, etc.