Shanghai Kekai Biotechnology Co., Ltd.

Genetic engineering production, no animal origin, no virus pollution, no heterozymatic pollution
1. Product introduction
Trypsin is a serine protease that cleaves peptide bonds at the C-terminus of lysine and arginine. Trypsin is widely used in various biotechnological processes. Such as: protein enzymatic hydrolysis to produce peptides; cell isolation of various tissues; Enzymatic hydrolysis and sequencing of proteins; Recombinant insulin production, etc. Recombinant porcine trypsin is produced by the expression of recombinant Escherichia coli, and the amino acid sequence is exactly the same as the cationic trypsin derived from the porcine pancreas. The specificity of digestion is the same. The molecular weight is 24kD, and the optimal pH is 7-10. Activity is inhibited by serine protease inhibitors such as PMSF, and metal ion chelators such as EDTA inhibit enzyme activity.
2. Product advantages
No animal origin: recombinant production, no exogenous virus contamination, such as swine influenza virus, porcine parvovirus, etc
High purity: no other heterozymatic contamination: no other side reactions of enzyme digestion reaction
Specific live height: not less than 3800 USP u/mg pro.
Stable: lyophilized powder, easy to store and transport; Mass production, stable product quality
3. Product use
Recombinant porcine trypsin has the same enzymatic properties as animal-derived porcine trypsin and can replace porcine pancreatic-derived trypsin in various biotechnological processes, such as: recombinant insulin production; Cell culture, cell fermentation; Enzymatic hydrolysis and sequencing of proteins; Cell isolation of various tissues, etc.